Swiftly attain sections as slim or thick as your application involves, without stressing about getting rid of or distorting precious tissue samples.
Megatome is really a vibrating microtome designed to part a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome permits superior-throughput tissue sectioning with uniform surface profile, in addition to minimum tissue injury and data reduction.
Antibodies could get weeks to diffuse by way of just a few millimeters of tissue, by using a steep labeling gradient from surface to Main.
Megatome is created for accuracy: the blade vibrates at a greater frequency and larger amplitude range than other microtomes, and encompasses a special deflection Command mechanism.
SE works by using a rotational electric powered subject to disperse very electromobile molecules (including antibodies or surfactant micelles) throughout a porous sample with no harming electrically charged structures within the tissue. This allows two-four day clearing of intact organs,
Our preformulated EasyIndex Alternative raises and homogenize the refractive index of delipidated tissue samples, rendering them entirely transparent. This allows light penetration in the sample and ensures the acquisition of large-resolution, in-concentration image facts.
Megatome is really a novel microtome which allows for prime-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with negligible tissue hurt and information decline.
It’s the perfect time to enhance your microtome to Megatome. With correct high-frequency slicing for an unmatched variety of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous apps.
The SmartSPIM light sheet microscope delivers unparalleled resolution and acquisition speed all over intact tissue volumes, although its modular structure permits you to upgrade and personalize unique factors.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
Leverage the Distinct+ tissue clearing system, as well as eFLASH and patented stochastic electrotransport systems, megatomi.com to rapidly distinct and label complete organs. Essential highlights and options include:
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